Composite

Part:BBa_K3002212:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + cCA_Mut-PETase_SP20HA + cCA_MHETase_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal NheI site found at 2665
    Illegal NheI site found at 5681
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
    Illegal NotI site found at 7094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal BglII site found at 8004
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5417
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6759
    Illegal PstI site found at 7083
    Illegal PstI site found at 7426
    Illegal PstI site found at 8236
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 5018
    Illegal NgoMIV site found at 6692
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002107 (cCA_Mutant PETase_SP20HA) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).

References